practical bacteriology from scratch
practical bacteriology from scratch | Udemy For tow days completely 100% Free
during everything you want to know you should know what will you do in lab
This course is written by the very popular author from Udemy Mohamed AL ashram The most recent Last updated 10/2021. The language of this course is
English, but also have sub-titles (captions) in English [US] languages to
better understand. This course is shared under the categories Teaching &
Academics, Other Teaching & Academics, Biology
More than 200 students had already enrolled. in the practical bacteriology
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Is this course right for you?
If you are wondering what you will learn or what things this best Udemy
courses will teach you after getting courses Udemy free coupon. Learn
practical bacteriology from scratch | Udemy: Okay, here are a few things.
-
medical students study either medicine pharmacy dentistry veterinary
medicine science secondary school prepare themselves to study medicine or
become science students prepare themselves to study medicine or science
- any one interested of microbiology
Requirements Course:
- no prerequisite
Description Course:
practical bacteriology from scratch
your safety first take care when working with pathogenic
microorganisms wear coat when you are in lab and remove after
finish keep the lab disk for equipment only wear gloves when dealing
with microorganisms clean clean table before and after
test mask eye protect keep your hand away from mouth and eye and
nose use mechanical pipetting report the spill and disinfect the
area well wash hands after any procedure deal well with fire
smooth small one with towel large one use expenditure follow the
instructions included in lab safety manual be professional in handling
microscope there are many types with many uses bright field
microscope dark field microscope phase contrast microscope
fluorescent microscope electro microscope it is essential tool
practice using the microscope with low power medium power high
power and take care when use oil immersion lens sterilization the most
important topic in microbiology without keeping your environment
sterile it is impossible to obtain Apure culture sterilization
methods heat sterilization sterilization by radiation
sterilization by disinfectant chemicals use of
antimicrobial bacterial culture is a method for isolate bacteria
the culture may be natural in origin or synthetic or both culture may
be ordinary enriched blood agar is enriched media
They contain substances Blood ,Serum. Extract of plants or animal
tissues that enhance growth of bacteria Heated blood agar
chocolate agar ▪ heated blood agar chocolate agar heating blood ▪ in
water path at 75c for 10 minutes ▪ Until blood become chocolate brown
in color differential or specific and see it
in colonies which in appearance characteristic after culture we need
to sea the bacterial cell clearly stain our tool to change the
background and sea bacterial morphology and enhance the contrast we
sea details differential or structural preparing the smear and
fix on Bunsen flame simple stain like methylene blue
crystal violet negative staining is simplest method
differential stain differentiate the bacteria into two
groups like gram stain differentiate bacteria into gram +ve gram
-ve it is invented by Hans gram he use methylene blue as basic
stain iodine as bacteria are not decolorized and remain
violet after decolorization carol fuchsine a red counter stain is used
to impart a pink color to the decolorized gram negative organisms The
acid fast stain is a different stain in 1882 Paul Ehrlich discovered
mycobacterium tuberculosis the causative agent of tuberculosis
retained the primary stain even after washing with an acid alcohol
mixture most bacteria are decolorized by acid alcohol only family
mycobacteriacae and nocardiacea The cell wall of acid fast contain wax
like lipid called mycolic acid render the cell impermeable to most
stain technique developed by Franz zeal and Fredrich nelson capsule
stain Some bacteria are surrounded by slimy layer called capsule some
glycoprotein some polypeptide all appear water soluble heat fixed
smear destroy the capsule if not fixed slide from slide combine
negative and simple staining species of bacteria related to genera
bacillus or clostridium produce heat resistant structure called spores
resistance to chemicals and heat due to thick tough spore coat
Schaeffer- Fulton methods utilize malachite green to stain the
endospores and safranin or carol fuchsine to stain the vegetative
portion of the cell green spore contained in pink vegetative
bacilli
bacteria to continue its life and defend herself produce enzymes like
oxidases catalase coagulase we use these enzyme in identifying every
bacteria biochemical test are designed for this purpose step by step
you can be bacteriologist just keep going
What I am going to learn?
-
principals of lab safety using lab equipment specially
microscope
- sterilization method in lab principals how to apply
- bacterial culture methods bacterial stain methods
- biochemical test test results
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